Micro- and Nanoengineering of the Cell Surface

Micro- and Nanoengineering of the Cell Surface

by Jeffrey M Karp, Weian Zhao
Released May 2014
Publisher(s): William Andrew
ISBN: 9781455731558

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Book description

Micro- and Nanoengineering of the Cell Surface explores the direct engineering of cell surfaces, enabling materials scientists and chemists to manipulate or augment cell functions and phenotypes. The book is accessible for readers across industry, academia, and in clinical settings in multiple disciplines, including materials science, engineering, chemistry, biology, and medicine. Written by leaders in the field, it covers numerous cell surface engineering methods along with their current and potential applications in cell therapy, tissue engineering, biosensing, and diagnosis.

The interface of chemistry, materials science, and biology presents many opportunities for developing innovative tools to diagnose and treat various diseases. However, cell surface engineering using chemistry and materials science approaches is a new and diverse field. This book provides a full coverage of the subject, introducing the fundamentals of cell membrane biology before exploring the key application areas.

  • Demystifies the direct engineering of cell surfaces, enabling materials scientists and chemists to manipulate or augment cell functions and phenotypes
  • Provides a toolkit of micro- and nanoengineering approaches to the manipulation of the cell surface
  • Unlocks the potential of cell surface manipulation for a range of new applications in the fields of in vitro research, cell therapy, tissue engineering, biosensing, and diagnostics

Table of contents

  1. Cover image
  2. Title page
  3. Copyright
  4. Foreword
  5. List of Contributors
  6. Chapter 1. Cell Membrane Biology and Juxtacrine Signal Conversion
    1. 1.1 Introduction
    2. 1.2 Cell Membrane Biology—Early Milestones
    3. 1.3 Membrane Microdomains
    4. 1.4 Cell Membrane Emergence
    5. 1.5 Juxtacrine Signaling and Rewiring Cellular Networks
    6. 1.6 Protein Painting, Artificial Veto Cell Engineering
    7. 1.7 Trans Signal Conversion
    8. 1.8 Redirecting Juxtacrine Signals
    9. 1.9 Creating Auto-Signaling Loops
    10. 1.10 SCP Therapeutic Flexibility
    11. 1.11 The Cell Membrane Frontier
    12. References
  7. Chapter 2. Cell Surface Engineering by Chemical Reaction and Remodeling
    1. 2.1 Introduction
    2. 2.2 Methods and Technology of Covalent Cell Surface Reaction
    3. 2.3 Relevance of Covalent Cell Surface Modification
    4. 2.4 Future Perspectives
    5. 2.5 Conclusions
    6. References
  8. Chapter 3. Bioconjugation Reactions in Living Cells: Development, Advances, and Applications of Glycan-Specific Technologies
    1. 3.1 Introduction
    2. 3.2 Bioorthogonal Chemical Ligation Reactions for Glycan Labeling
    3. 3.3 Bioorthogonal Ligation Reactions: Exploitation in MOE-Based Applications
    4. 3.4 Concluding Comments
    5. Acknowledgments
    6. References
  9. Chapter 4. Pushing the Bacterial Envelope: Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins and Sugars
    1. 4.1 Bacterial Surface Display
    2. 4.2 Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins
    3. 4.3 Applications of Bacteria Expressing Heterologous Surface Proteins
    4. 4.4 Strategies for Re-Engineering Bacterial Surfaces with Heterologous Sugars
    5. 4.5 Applications of Bacteria Expressing Heterologous Surface Sugars
    6. 4.6 Conclusion
    7. Acknowledgment
    8. References
  10. Chapter 5. Noncovalent Functionalization of Cell Surface
    1. 5.1 Introduction
    2. 5.2 Methods of Cell Surface Engineering—Applications and Recent Developments
    3. 5.3 Electrostatic Interactions Mediated Cell Surface Modification
    4. 5.4 Advantages and Limitations of the Noncovalent Modification of the Cell Surface
    5. 5.5 Conclusions and Future Perspectives
    6. Acknowledgments
    7. References
  11. Chapter 6. Lipid-Mediated Cell Surface Engineering
    1. 6.1 Introduction
    2. 6.2 One-Step Protein Transfer
    3. 6.3 Two-Step Protein Transfer
    4. 6.4 Summary
    5. Acknowledgments
    6. References
  12. Chapter 7. Engineering the Surface of Cells Using Biotin–Avidin Chemistry
    1. 7.1 Introduction: Rationale for Engineering the Cell Surface
    2. 7.2 Biotin and Avidin: An Overview
    3. 7.3 Methods for Engineering Cell Surfaces with Avidin–Biotin Complexes
    4. 7.4 Applications of Cell Surface Engineering Using Avidin–Biotin Chemistry
    5. 7.5 Conclusion
    6. References
  13. Chapter 8. Construction and Computation with Nucleic Acids on the Cell Surface
    1. 8.1 Computation of Cell Identity
    2. 8.2 Targeted Transport of a Payload to a Cell Surface and Activation of Innate Cellular Response
    3. 8.3 DNA Channels and Pores Spanning Lipid Membrane
    4. 8.4 Advances in Microscopy
    5. 8.5 Conclusion
    6. References
  14. Chapter 9. Cell Surface Enzymatic Engineering-Based Approaches to Improve Cellular Therapies
    1. 9.1 Introduction
    2. 9.2 Use of Enzymes to Modify Cell Surface Carbohydrates on Proteins and Lipids to Enhance Migration to Tissues
    3. 9.3 Modification of Enzyme Activity Leads to Changes on Cell Surface Structures that Deter Migration and Metastasis
    4. 9.4 Use of Enzymes to Improve the Therapeutic Function of Cells Through Modification of the Cell Surface
    5. 9.5 Conclusion
    6. References
  15. Chapter 10. Cell Microencapsulation for Tissue Engineering and Regenerative Medicine
    1. 10.1 Introduction
    2. 10.2 Encapsulation Requirements and Strategies
    3. 10.3 Materials Used for Cell Encapsulation
    4. 10.4 Therapeutic Applications of Encapsulated Cells
    5. 10.5 Challenges and Future Perspectives
    6. Acknowledgments
    7. References
  16. Chapter 11. Cell Engineering with Nanoparticles for Cell Imaging
    1. 11.1 Introduction
    2. 11.2 Imaging Modalities for NP Engineered Cells
    3. 11.3 Strategies for Cell Engineering with NPs
    4. 11.4 Challenges and Outlook
    5. 11.5 Conclusion
    6. Acknowledgments
    7. References
  17. Chapter 12. Micro/Nano-Engineering of Cells for Delivery of Therapeutics
    1. 12.1 Cell Therapy—Success and Current Challenges
    2. 12.2 Cell Surface Engineering to Improve Cell Targeting
    3. 12.3 Cell-Based Drug Delivery
    4. 12.4 Concluding Remarks
    5. References
  18. Chapter 13. Molecular Engineering of Cell and Tissue Surfaces with Polymer Thin Films
    1. 13.1 Introduction
    2. 13.2 General Design Principles and Considerations
    3. 13.3 Cell Surface Engineering with Polymer Thin Films
    4. 13.4 Biomedical Applications
    5. 13.5 Conclusion
    6. References
  19. Chapter 14. Biofunctionalization of Hydrogels for Engineering the Cellular Microenvironment
    1. 14.1 The 3D Extracellular Milieu
    2. 14.2 Mimicking the ECM
    3. 14.3 Engineering Degradability into Hydrogels
    4. 14.4 Hydrogel Nanoparticles
    5. 14.5 Conclusion
    6. List of Abbreviations
    7. References
  20. Chapter 15. Probe and Control of Cell–Cell Interactions Using Bioengineered Tools
    1. 15.1 Introduction
    2. 15.2 In Vitro Study of Cell–Cell Interactions Using Engineered Microdevices
    3. 15.3 Probing and Manipulation of Cell–Cell Interactions Using Engineered Molecular Tools
    4. 15.4 Conclusions and Perspectives
    5. References
  21. Index

Product information

  • Title: Micro- and Nanoengineering of the Cell Surface
  • Author(s): Jeffrey M Karp, Weian Zhao
  • Release date: May 2014
  • Publisher(s): William Andrew
  • ISBN: 9781455731558