Pushing the Bacterial Envelope
Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins and Sugars
Samir Gautam1 and David A. Spiegel2, 3, 1Department of Cell Biology, Yale School of Medicine, New Haven, CT, USA, 2Department of Pharmacology, Yale School of Medicine, New Haven, CT, USA, 3Department of Chemistry, Yale University, New Haven, CT, USA
Over the past three decades, a powerful array of techniques has been developed for expressing heterologous proteins and saccharides on the surface of bacteria. Surface-engineered bacteria, in turn, have proven useful in a variety of settings, including high-throughput screening, biofuel production, and vaccinology. In this chapter, we provide a comprehensive ...
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