Cell Engineering with Nanoparticles for Cell Imaging
David C. Yeo, Riddhima Minocha and Chenjie Xu, Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore
Genetic engineering of cells is a popular approach to create permanent labels for cells (e.g., the green fluorescent protein). However, it is limited by labeling efficiency, technical complexity, and crucially, the risk of incorporating random mutations which makes them unsuitable in the clinic. Nanoparticle (NP) engineering, an alternative to genetic engineering, is similarly able to incorporate imaging labels on cells at a high efficiency while being less detrimental to the cell. This chapter gives an overview ...
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