Microscope Image Processing

to locate a certain percentile point, I

p

. Using the symmetry assumption, the

threshold value is then selected on the other side of the background peak at an

equal displacement from the background peak; that is,

T ¼ I

max

 (I

p

 I

max

)(9:5)

This algorithm can easily be adapted to the case where the object peak dominates

instead of the background peak and is approximately symmetrical.

The Triangle Algorithm The triangle algorithm is known to be

particularly effective when the object pixels produce a weak peak in the

histogram [17], as illustrated in Fig. 9.3, where low gray-level objects reside on

a high gray-level background. One first finds the maximum