Chapter 6Hydrodynamic Flow Confinement-Assisted Immunohistochemistry from Micrometer to Millimeter Scale

Robert D. Lovchik, David P. Taylor, Emmanuel Delamarche and Govind V. Kaigala

IBM Research – Zurich, Säumerstrasse 4, 8803 Rüschlikon, Switzerland

In this chapter, we outline the importance of immunohistochemical analysis applied to tissue sections and review the state-of-the-art IHC implementations, in particular those using a microfluidic probe. We demonstrate the efficacy and applicability of specific probe head designs in the context of surface assays by performing uniform and multiplexed immunohistochemistry on tissue sections in the micrometer to millimeter scale.

6.1 Immunohistochemical Analysis of Tissue Sections

IHC, a staining ...

Get Open-Space Microfluidics now with O’Reilly online learning.

O’Reilly members experience live online training, plus books, videos, and digital content from 200+ publishers.