July 2012
Intermediate to advanced
758 pages
17h 12m
English
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Chapter 1
Paper Chromatography
INTRODUCTION
The first chromatograph was invented by Mikhail Semenovich Tsvett. Again it was proposed by the Richard Martin. First, the scientists discovered that the filter paper is used as a substitute for column absorbing powder. Paper chromatography theory was first given by the Archer John porter Martin and Richard Laurence Millington Synge. Later Frederick Sanger applied this method for the determination of insulin structure. Then Melvin Calvin used this technique for the determination of energy producing cells. Watson and Crick used for determination of the DNA structure.
PRINCIPLE
This method is mainly used for the analysis, identification, purification and quantification of mixtures of components into individual ...