July 2012
Intermediate to advanced
758 pages
17h 12m
English
Content preview from Pharmaceutical Analysis
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Chapter 2
Column Chromatography
INTRODUCTION
Matteucci first described the column use in the determination of the solutes in the solvents. Kuhn and Lederer explained the column chromatography theory by observing the separation of the polyene pigments.
PRINCIPLE
The main principle involved in column chromatography is adsorption of the solutes of a solution through a stationary phase and separates the mixture into individual components. This is based on the affinity towards the mobile phase and stationary phase. The molecules which are more affine towards the stationary phase elute later and which are less affine towards the stationary phase elutes first.
Separation by the column chromatography
THEORY
The sample solution of the concentration ...