Matteucci first described the column use in the determination of the solutes in the solvents. Kuhn and Lederer explained the column chromatography theory by observing the separation of the polyene pigments.
The main principle involved in column chromatography is adsorption of the solutes of a solution through a stationary phase and separates the mixture into individual components. This is based on the affinity towards the mobile phase and stationary phase. The molecules which are more affine towards the stationary phase elute later and which are less affine towards the stationary phase elutes first.
Separation by the column chromatography
The sample solution of the concentration ...