July 2012
Intermediate to advanced
758 pages
17h 12m
English
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Chapter 3
Thin Layer Chromatography
INTRODUCTION
In 1949, Mein hard and Hall first proposed Thin Layer Chromatography (TLC) method by using the starch as binder to separate the inorganic ions. Kirchner proposed the conventional ascending TLC method. TLC is a solid-liquid technique in which two phases are there: one is stationary phase and other is mobile phase. The TLC principle was first introduced by the Izmailov and Shraiber. Stahl was first designed the standard equipment for the TLC. It is similar to that of the paper chromatography.
PRINCIPLE
The main principle involved in the TLC is the mobile phase flows through the stationary phase where a solid or liquid is supported on the solid and carries the components of mixture which will be separated ...
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