July 2012
Intermediate to advanced
758 pages
17h 12m
English
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Chapter 10
Nano Flow Liquid Chromatography
INTRODUCTION
The field of proteomics has experienced a fast evolution in recent years. Unlike the analysis of nucleic acids, there is not an equivalent method to polymerase chain reaction for the amplification of proteins. Thus the methodologies for proteomics have to be successfully sensitive to be able to detect endogenous levels of proteins. Due the analytical demands of biological samples, miniaturised liquid chromatography (LC) techniques were developed to allow for analysis of samples with greater sensitivity than that afforded by conventional LC, one of these methods, namely nano flow LC, with emphasis on studies that used it in combination with MS/MS.
PRINCIPLE
The main principle used in nano ...
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