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Droplet-Based Microfluidics for Biological Sample Preparation and Analysis

Xuefei Sun and Ryan T. Kelly

CONTENTS

1.1    Introduction

1.2    Droplet-Based Operations

1.2.1    Droplet Generation

1.2.2    In-Droplet Reagent Combination and Mixing

1.2.3    Droplet Readout Strategies

1.3    Perspectives for Droplet-Based Microfluidics

1.3.1    Enhanced LC/MS-Based Proteomic Analysis

1.3.2    Single-Cell Chemical Analysis

1.4    Conclusions

References

1.1  INTRODUCTION

Modern biological research often requires massively parallel experiments to analyze a large number of samples in order to find biomarkers, screen drugs, or elucidate complex cellular pathways. These processes frequently involve time-consuming sample preparation and expensive biochemical ...

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