Chapter 8: Recent approaches and innovations for enzyme engineering used in industrial biotechnology
Charu Tripathi, and Twinkle Yadav
Department of Zoology, CMP College, University of Allahabad, Prayagraj, Uttar Pradesh, India
Abstract
Industrial biotechnology has largely relied on microbial enzymes due to ease of cultivation, manoeuvrability, and high yield. In order to achieve optimal activity and target yield, a number of strategies such as directed evolution, enzyme promiscuity, enzyme immobilization, and strain engineering have been deployed. The promiscuous activity of several enzymes has been investigated, especially with the aid of computational tools, leading to the discovery of novel production routes and substrates for ...
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