Next-Generation Sequencing Data Analysis, 2nd Edition

Appendix II392

Paired- End Reads: Reads obtained from the two ends of a DNA fragment.

Since the length of the DNA fragment, i.e., the distance between the

reads, is known, use of paired- end reads provides additional positional

information in mapping or assembly of the reads. In comparison to

Single- End Reads.

Pathway: A succession of molecular events that leads to a cellular response

or product. Each of such events is usually carried out by a gene

product. Many biological pathways are involved in metabolism, signal

transduction, and gene expression regulation.

PCA: Principal Component Analysis. A dimensionality reduction technique

to help summarize and visualize large and complex datasets. PCA is

widely used in next- gen sequencing applications ...