February 2016
Intermediate to advanced
452 pages
16h 17m
English
Content preview from Tunable Laser Applications, 3rd Edition
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Chapter 9
Tunable Laser Microscopy
F. J. Duarte
9.1 Introduction
A laser microscope is a microscope optical system that uses a laser as the illumination source. Very briefly, lasers were introduced to the field of microscopy in the mid-1960s [1], and confocal scanning laser microscopes were disclosed in the late 1960s [2,3]. The concept of scanning meant the displacement of the laser beam cross section over the specimen to be observed [4]. An additional illumination approach in scanning laser microscopy is near-field laser illumination [5,6].
Static, or instantaneous, scanning using extremely elongated Gaussian beams, yielding illumination very wide in one optical axis while very thin in the orthogonal axis, produced via beam expander optics, ...
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