Microfluidics and Nanotechnology

4 Measuring the Physical Properties of Cells

Shirin Mesbah Oskui and William H. Grover

CONTENTS

4.1 Introduction

4.1.1 The Importance of Water

4.1.2 The Importance of Cell Size

4.1.3 Sorting versus Measuring

4.2 Measuring Cell Volume

4.2.1 The Promise of Microfluidic Coulter Counters

4.2.2 Increasing Dynamic Range

4.2.3 Increasing Throughput

4.2.4 Low-Cost Coulter Counters

4.2.5 Sensitivity of Coulter Counter Volume Measurements to Aperture Geometry and Cell Shape/Orientation

4.3 Measuring Cell Mass

4.3.1 Suspended Microchannel Resonators

4.3.2 Measuring the Mass of Single Cells

4.3.3 Measuring Cell Growth

4.4 Measuring Cell Density

4.4.1 Measuring Average Cell Density

4.4.2 Measuring Single-Cell Density

4.4.3 Mass-Based Measurement of Cell ...