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Design of Experiments with Several Factors

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Carotenoids are fat-soluble pigments that occur naturally in fruits in vegetables and are recommended for healthy diets. A well-known carotenoid is beta-carotene. Astaxanthin is another carotenoid that is a strong antioxidant and commercially produced. An exercise later in this chapter describes an experiment in Biotechnology Progress to promote astaxanthin production. Seven variables were considered important to production: photon flux density and concentrations of nitrogen, phosphorous, magnesium, acetate, ferrous, and NaCl. It was important to study the effects of these factors as well as the effects ...

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