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all histomorphometric parameters. To verify this
fact, we applied a paired t-test on the μCT images
segmented by the three methods. The results pre-
sented in Table 2 showed that for the L3 vertebra,
OTH and MTH were able to distinguish adult and
young groups with four histomorphometric param-
eters, whereas DTH could distinguish only with
three parameters. When considering L4 vertebra,
the t-test showed that OTH and DTH could differ-
entiate both the groups with two parameters, while
MTH could not find any difference. Thus, accord-
ing to these results, OTH has the best perform-
ance in distinguishing young and adult vertebra.
Additionally, it is important to take into account
that MTH is an operator-dependent method, and
hence, the results ...