Biophysics and Molecular Biology: Tools and Techniques by Pearson

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Electrophoresis

Electrophoresis (Electro refers to the energy of electricity and Phoresis, from the Greek verb phoros, means to carry across) is a technique for separating or resolving charged molecules (such as amino acids, peptides, proteins, nucleotides, and nucleic acids) in a mixture under the influence of an applied electric field. Charged molecules in an electric field move or migrate, at a speed determined by their charge to mass ratio. According to the laws of electrostatics, an ion with charge ‘Q’ in an electric field of strength ‘E’ will experience an electric force, F_electrical

The resulting migration of the charged molecule through ...