12
Molecular biology techniques
12.1 Nucleic acid extraction
The extraction of nucleic acid is a crucial step for biochemical and diagnostic processes. It is a series of steps to obtain pure nucleic acid samples that are suitable for different downstream applications. Different extraction methods result in the difference in yield, purity and extraction time.
DNA extraction
The choice of the DNA extraction method depends on the types of DNA (chromosomal and plasmid), source organism (bacteria, fungi, plant or animal), starting material (organ, tissue, cell, etc.) and desired results (molecular weight of the desired DNA, purity, extraction time required, etc.).
The extraction of DNA from biological material requires: cell lysis (cell disruption), ...
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