CHAPTER 18Designing PCR Primers Using the Primer3 Online Tool
CS Mukhopadhyay and RK Choudhary
School of Animal Biotechnology, GADVASU, Ludhiana
18.1 INTRODUCTION
The previous chapter (Chapter 17) discussed in brief the salient features of PCR‐primers. Here we will learn how to use Primer3, an online software tool, for designing PCR primers.
18.2 OBJECTIVE
To design primers for bubaline Dicer I cds, using the online primer designing tool Primer3.
18.3 PROCEDURE
18.3.1 Downloading a nucleotide sequence
Type the URL (www.ncbi.nlm.nih.gov/) to open the NCBI home page. Search the required nucleotide sequence, select the target sequence from NCBI nucleotide and save it in FASTA format in a text file.
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