Bioinformatics and Biomedical Engineering: New Advances

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To further verify the synergistic and antagonistic actions between 5 mmol/l 4-AP with

5-FU and 5 mmol/l 4-AP with paclitaxel were related to cell cycle, we used flow cytometry

to examine the cell cycle progression of MCF-7 cells treated with 5-FU or paclitaxel in the

presence or absence of 4-AP. The results were depicted in Figure 5. Compared to the cells

treated with 5-FU only, the number of cells arrested at the S phase was significantly increased

when the cells were pre-treated and co-administrated with 5 mmol/l 4-AP. Figure 5 also shows

that paclitaxel induced apparent arrest at G

2

/M phase, but this paclitaxel-induced arrest was

clearly blocked when the cells were pre-treated and co-administrated with 5 mmol/l 4-AP,

indicating tha