Bioinformatics and Biomedical Engineering – Chou & Zhou (Eds)
© 2016 Taylor & Francis Group, London, ISBN 978-1-138-02784-8
257
Optimization of protein depletion technology for polysaccharides
from Angelica and Astragalus by orthogonal test design
X.Y. Pu, X.L. Ma, L. Liu, J. Ren, X.Y. Li & H.B. Li
College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, China
ABSTRACT: In this study, we developed and optimized an efficient enzyme-assisted protein
depletion procedure for polysaccharides from Angelica and Astragalus. The Percentage of the
Removed Protein (PRP) served as an evaluation index to compare the deproteinization effects
of the Sevag method, TCA method and enzyme-assisted method. Processing parameters f ...