10.9

Confocal Microscopy

Fatima A. Merchant,     Advanced Digital Imaging Research

Keith A. Bartels,     Southwest Research Institute

Alan C. Bovik and Kenneth R. Diller,     The University of Texas at Austin

1 Introduction

2 Image Formation in Confocal Microscopy

2.1 Lateral Resolution

2.2 Depth Resolution and Optical Sectioning

3 Confocal Fluorescence Microscopy

4 Further Considerations

5 Types of Confocal Microscopes

5.1 Scanning Confocal Microscope

5.2 The Tandem Scanning Optical Microscope

6 Limitations of Confocal Microscopy

6.1 Two-Photon Laser Scanning Confocal Microscopy

7 Biologic Applications of Confocal Microscopy

7.1 Quantitative Analysis of 3D Confocal Microscope Images

7.2 Cells and Tissues

7.3 Microvascular Networks ...

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