Chapter 14. Extracting, Isolating, and Visualizing DNA

Equipment and Materials

You’ll need the following items to complete this lab session. (The standard kit for this book, available from www.thehomescientist.com, includes the items listed in the first group.)

Materials from Kit

  • Goggles

  • Centrifuge tubes, 50 mL

  • Coverslips

  • Eosin Y stain

  • Funnel

  • Graduated cylinder, 10 mL

  • Methylene blue stain

  • Microscope slides (flat)

  • Pipettes

  • Reaction plate, 96-well

  • Scalpel

  • Sodium dodecyl sulfate, 10%

  • Spatula

  • Stirring rod

  • Test tubes

  • Test tube rack

  • Yeast (optional)

Materials You Provide

  • Gloves

  • Balance (optional)

  • Beef or pork liver, raw (or yeast)

  • Cheesecloth (or muslin, etc.)

  • Isopropanol (see text)

  • Freezer

  • Microscope

  • Paper towels

  • Saucer

  • Table salt

  • Teaspoon

  • Toothpick

  • Water, distilled

Background

All organisms ...

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