
64 Green Chemical Engineering
(1/r
p
) versus (1/[S]) and calculating the slope (k
M
/k) and intercept (1/k) of the straight line,
the kinetic parameters k and k
M
can be estimated.
There are some enzyme-catalysed reactions in which the substrate [S] inhibits the for-
mation of product P by binding to the energised complex ES to form a complex ES
2
. The
mechanism of this reaction is represented as
ES ES P
ES SES
k
+
3
2
The formation of product P is the rate-controlling step, and the overall rate equation is
r
P
= k
3
[ES] (2.170)
Assuming the intermediate complexes ES and ES
2
to be in a pseudo-equilibrium state
(stationary state), we can write the equations ...