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Essentials of Molecular Biology by Malathi V.

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12

Techniques In Molecular Biology

CONCEPT OUTLINE
  • Introduction
  • Gel Electrophoresis
    • DNA electrophoresis
    • Protein electrophoresis
  • Blotting Techniques
    • Southern blotting
    • Northern blotting
    • Western blotting
  • Restriction Digestion and Ligation
    • Ligation

       

  • Random Amplification of Polymorphic DNA
    • Applications of RAPD analysis

       

  • Polymerase Chain Reaction
    • The PCR reaction components
    • PCR thermocycling
    • Types of PCR
  • Summary
  • References
INTRODUCTION

This chapter provides information regarding the practical side of molecular biology. Molecular biology methods are used extensively in modern-day drug discovery, research and development, and diagnostics. In the previous chapters too, various other techniques in molecular biology such as cloning, ...

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