Essentials of Molecular Biology

12

Techniques In Molecular Biology

CONCEPT OUTLINE

• Introduction
• Gel Electrophoresis
  • DNA electrophoresis
  • Protein electrophoresis
• Blotting Techniques
  • Southern blotting
  • Northern blotting
  • Western blotting
• Restriction Digestion and Ligation
  • Ligation

     

• Random Amplification of Polymorphic DNA
  • Applications of RAPD analysis

     

• Polymerase Chain Reaction
  • The PCR reaction components
  • PCR thermocycling
  • Types of PCR
• Summary
• References

INTRODUCTION

This chapter provides information regarding the practical side of molecular biology. Molecular biology methods are used extensively in modern-day drug discovery, research and development, and diagnostics. In the previous chapters too, various other techniques in molecular biology such as cloning, ...