High Value Fermentation Products, Volume 1

Chapter 4Antibiotic Against Gram-Negative Bacteria

Maryam Faiyaz¹, Shikha Gupta² and Divya Gupta^3*

¹Department of Bioengineering, Integral University, Lucknow, Dasauli, (India)

²Gujarat State Biotechnology Mission, Gandhinagar, (India)

³Department of Life sciences, Uttarakhand Technical University, Dehradun, (India)

*Corresponding author: [e-mail]

Abstract

Already available drugs in the market are unable to keep pace with the rate of production of novel bacterial strains in the environment. Antibiotics, if not used appropriately, give rise to resistant microorganisms. This has become an issue of great concern to scientists as well as medical practitioners, even more so if the bacteria is gram-negative. Efforts from biotechnologists and pharmacist in collaboration with scientists are being made to get large-scale production of antibiotics so that people can benefit from it when it is commercialized. The present study focuses on the steps involved in the fermentation of antibiotics acting against gram-negative bacteria. It also deals with the components and factors involved in the scaling up of antibiotics and the importance of quality control in the whole process. For antibiotic production, solid state fermentation (SSF) has been prioritized over submerged fermentation (SmF) which is discussed in this chapter. Techniques such as protoplast fusion, mutagenesis, recombinant DNA technology, etc., are widely in use to improve strain for better antibiotic yield. Selections ...