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Appendix A
APPENDIX A
NCBI Display Formats
NCBI-BLAST has several options for displaying sequence alignments. These options
are available for the five basic BLAST programs (BLASTN, BLASTP, BLASTX,
TBLASTN, TBLASTX), PSI-BLAST, PHI-BLAST, and MegaBLAST. For all pro-
grams, these formats are selected by using the –
m option; however, in MegaBLAST,
the formats must first be set with the –
D2option to use classic BLAST formatting.
The next section gives a brief description of each option, followed by a detailed
explanation and an example.
Brief Descriptions
The alignment display format is set with the –m option followed by a number from 0
to 11 as you can see in the following table.
Detailed Descriptions and Examples
This section includes detailed descriptions of each format, followed by an example.
To create the examples, the authors performed a BLASTP search of the coelacanth
HoxA11 protein sequence (AAG39070) versus the HoxDB.pep database, which is
included in the online supplement.
Option number Brief description
0 The default pairwise display. Classic BLAST format.
1-6 Various types of query-anchored multiple sequence alignments. The query is anchored and the aligned
regions of the subjects are displayed underneath.
7 eXtensible Markup Language (XML) output.
8 Tabular output, without header ...