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Chapter 10
CHAPTER 10
Installation and Command-Line
Tutorial
This chapter shows you how to install NCBI-BLAST and WU-BLAST software on
your own computer. This is necessary if you want to use BLAST in a high-through-
put setting or develop specialized applications. After the installation section, this
chapter presents a command-line tutorial that also serves as a test suite to make sure
your BLAST installation behaves as expected.
NCBI-BLAST Installation
NCBI-BLAST, as the name implies, is available from the National Center for Biotech-
nology Information (NCBI). Precompiled binaries and source code are available for
free and without restriction. The source code is in the public domain, so there are
quite a few derivative works, both commercial and free (see Chapter 12). NCBI-
BLAST is currently available as precompiled binaries for 11 popular operating sys-
tem-hardware combinations. In addition, there is this very generous statement in the
README.bls file:
BLAST binaries are provided for IRIX6.2, Solaris2.6 (Sparc) Solaris2.7 (Intel), DEC
OSF1 (ver. 5.1), LINUX/Intel, HPUX, AIX, BSD Unix, Darwin, MacIntosh, and
Win32 systems. We will attempt to produce binaries for other platforms upon request.
If you have a platform that isn’t supported as a precompiled binary, you may wish to
take