July 2003
Intermediate to advanced
368 pages
13h 44m
English
Content preview from BLAST
Become an O’Reilly member and get unlimited access to this title plus top books and audiobooks from O’Reilly and nearly 200 top publishers, thousands of courses curated by job role, 150+ live events each month,
O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.
This is the Title of the Book, eMatter Edition
Copyright © 2012 O’Reilly & Associates, Inc. All rights reserved.
130
Chapter 9
CHAPTER 9
BLAST Protocols
This chapter contains protocols for the most common BLAST searches. Because
every BLAST experiment is unique, you should treat the protocols as a starting point
and use your own knowledge about BLAST to modify the procedures. The discus-
sions include what to do, as well as why. Although this approach makes the descrip-
tions more verbose, explaining the logic behind these choices will help you make
intelligent choices when creating your own protocols.
Most BLAST experiments fall into one of two categories: mapping and exploring.
Mapping is the process of finding the position of one sequence within another—for
example, finding a gene within a genome. When mapping, you can expect the align-
ments to be nearly identical, and the coordinates are generally the focus of the results.
When exploring, the goal is usually to find functionally related sequences. When
exploring, your alignment statistics (score, expectation, percent identity, etc.) are
often of greatest importance, at least initially. Making functional and phylogenetic
inferences, especially between distantly related sequences, often requires inspecting
the alignments from a biological rather than a statistical perspective. There is, of
course, a continuum between mapping and exploring, but ...
Become an O’Reilly member and get unlimited access to this title plus top books and audiobooks from O’Reilly and nearly 200 top publishers, thousands of courses curated by job role, 150+ live events each month,
and much more.
Read now
Unlock full access
More than 5,000 organizations count on O’Reilly
O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.Julian F.
I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.Addison B.
I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.Amir M.
I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.