
This is the Title of the Book, eMatter Edition
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Using NCBI-BLAST
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Graphical Overview option) as they align to the query. At the top of the display, you
can see that 72 BLAST hits passed the threshold of your search criteria (you may see
more than 72 because of the rapid database growth). After the color key, the top line
represents the query sequence as a solid red line with the sequence coordinates. Each
line below represents one subject match with its position in relation to the query and
the color-coded relative strength of the similarity. You can move your mouse over
each line to see the definition line, and if you click on it, you will be taken to the
actual alignment.
The next part of the body is the summary (see Figure 1-9), which lists the one-line
descriptions (set with the Descriptions option) of the database matches (also known
as hits or subjects) along with the score and the E value. The hits are listed from best
to worst, with high scores and low E values being better. Also included in this part,
and set with the Linkout option, are links to other NCBI curated databases with
more information about each hit. In this case some sequences have links in Locus-
Link (L) and/or UniGene (U).
At the heart of the report are the actual alignments (the number of alignments dis-
played is controlled by the Alignments option). The ...