July 2003
Intermediate to advanced
368 pages
13h 44m
English
Content preview from BLAST
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Chapter 1: Hello BLAST
Using NCBI-BLAST
This book begins by exploring the BLAST pages on the NCBI web site. The NCBI,
part of the National Institutes of Health, is a U.S. government-funded center for the
curation and presentation of public biological knowledge. The NCBI is a public
repository for DNA and protein sequences (GenBank), but it’s far more than just a
data storehouse. The NCBI also maintains a comprehensive medical publication
archive (PubMed), distributes many tools for biological analyses (NCBI toolbox),
and puts together its own tools for making the most use of the data that it stores
(LocusLink, UniGene, RefSeq, Taxonomy browser). Most importantly, for our pur-
poses, it’s where the BLAST algorithm was first developed (Altschul et al., 1990) and
where it can be obtained, distributed, and used for free without restrictions. Anyone
with access to the Internet can run a BLAST search and explore the plethora of
genetic resources that have been amassed and curated by the NCBI over the years.
You’ll get the most out of this chapter if you follow along with a web browser. Begin
by going to the BLAST homepage at http://www.ncbi.nlm.nih.gov/BLAST.
Choosing the BLAST Program
Without explaining all of the options presented on the homepage, let’s get right into
it with a default
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I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.Addison B.
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I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.