January 2015
Intermediate to advanced
412 pages
12h 10m
English
Content preview from Electrochemical Biosensors
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November 11, 2014 15:47 PSP Book - 9in x 6in 01-Serge-Cosnier-c01
26 Electrochemical Detection of Exocytosis
amperometry may detect and analyze exocytotic events only after
the initial fusion pore between vesicles/cells has been formed.
Conversely, optical techniques like TIRF microscopy, though not
quantitative, are informative about such prefusion mechanisms.
Hence, ideally, coupling such optical methods with amperometry
should afford the comprehensive total life of a fusing vesicle from
its trail in the cytoplasm up to fusion. Although both methodologies
operate on single cells, experiments have to be repeated on
many cells to diminish the biases
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