January 2015
Intermediate to advanced
412 pages
12h 10m
English
Content preview from Electrochemical Biosensors
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November 7, 2014 13:15 PSP Book - 9in x 6in 09-Serge-Cosnier-c09
272 Conductometric Enzyme Biosensors
maltose detection by a conductometric biosensor is as follows:
α-Glucosidase
Maltose + H
2
O → α-D-glucose + α-D-glucose (9.17)
Mutarotase
α-D-glucose → β-D-glucose
Glucose oxidase (9.18)
β-D-glucose + O
2
→ D-gluconolacton + H
2
O
2
⇓ (9.19)
D-gluconic acid + H
2
O Acid residue + H
+
The calibration curve of a maltose conductometric biosensor is
shown in Fig. 9.13. The linear range of biosensor operation was up
to 1 mM, and the minimum measurable concentration was 0.002 mM
for maltose.
Selectivity as an essential characteristic of the conductometric
maltose biosensor ...
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