
November 11, 2014 15:51 PSP Book - 9in x 6in 07-Serge-Cosnier-c07
Applied Technologies 215
500 cfu/mL of E. coli cells, without the nucleic acid amplification
step, using Fe
2
O
3
@Au core–shell NPs and a probe modified with
HRP in a sandwich assay. The Fe
2
O
3
@Au core–shell NPs have
two functions, functionalization with the capture sequence through
gold and magnetic separation, thanks to the Fe
2
O
3
paramagnetic
properties. The amperometric detection of the enzymatic reaction of
reduction of 2,2
-diaminoazobenzene catalyzed by HRP was finally
detected.
Another enzyme was used by Chen et al. [59]. The authors
developed a biosensor based on a sandwich assay and the ...