January 2015
Intermediate to advanced
412 pages
12h 10m
English
Content preview from Electrochemical Biosensors
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November 7, 2014 13:14 PSP Book - 9in x 6in 08-Serge-Cosnier-c08
228 Electroenzymatic Labeling for Immunosensors and DNA Sensors
by coupling with the bioelectrocatalytic process of adenosine
deaminase and ALP [9]. Adenosine deaminase and IgG were initially
immobilized on the ammonium ISE, and then the signal antibodies
were employed by using anti-IgG–protein A–ALP conjugates. With
the competitive-type immunoassay mode, the immobilized IgG on
the electrode and the analyte (IgG) competed with ALP-conjugated
anti-IgG antibodies. Therefore, the conjugation amount of ALP-
anti-IgG on the electrode decreased with the increase of IgG
concentration. Upon addition ...
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