January 2015
Intermediate to advanced
412 pages
12h 10m
English
Content preview from Electrochemical Biosensors
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November 7, 2014 13:15 PSP Book - 9in x 6in 12-Serge-Cosnier-c12
352 Biosensors Based on Electrochemiluminescence
dissolved oxygen to produce equimolar hydrogen peroxide, the
ECL intensity decreases. This strategy can be applied in more
bioanalytical systems for oxidase substrates.
Scheme 12.9:
CdSe/Cd(TGA) QDs + ne → nR
•−
(12.36)
Catalyzed by GOD: Glucose + O
2
→ Gluconate + H
2
O
2
(12.37)
For oxygen : O
2
+ 2R
•−
+ 2H
+
→ 2R
∗
+ H
2
O
2
(12.38)
H
2
O
2
+ 2R
•−
→ 2R
∗
+ 2OH
−
(12.39)
For peroxide : H
2
O
2
+ 2R
•−
→ 2R
∗
+ 2OH
−
(12.40)
Then R
∗
→ R + hν (12.41)
With the same mechanism, a disposable ECL biosensor has been
fabricated by co-immobilizing GOD and surface-unpassived CdTe
QDs on a
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