June 2014
Intermediate to advanced
502 pages
17h 39m
English
Content preview from Biomaterials for Bone Regeneration
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384 Biomaterials for Bone Regeneration
transfer genes ef ciently to both quiescent and proliferating cells. The main
disadvantages of AAV vectors are the small insert size they can accommo-
date and the use of helper viruses in the manufacturing process, which has
caused problems, such as low titer, contamination, and costly puri cation
procedures. However, progress has been made in the manufacturing pro-
cess, which allows high-titer production without helper viruses.
12.3.2 Non-viral vectors
Viral vectors have been shown to be ef cient gene transfer tools.
Nevertheless, drawbacks, such as the bloodstream’s rapid clearance of viral
vectors (when injected systemically), their immunogenic and in ammatory
potential, together with certain ...