Biomaterials for Bone Regeneration

Stem cell technology for in vivo bone repair 439

require animal sacri ce and taking out the tissue samples. By using certain

threshold settings, it is usually possible to distinguish between scaffold and

newly formed bone. Micro-CT can subsequently be combined with histo-

morphometric analysis of Masson trichrome-stained tissue sections of the

same samples for quanti cation of bone formation parameters, as elegantly

shown in Farr é -Guasch et al . (2012). Histology can also be applied to iden-

tify the transplanted cells labeled for cell tracking.

14.5 Safety considerations and regulations

In the past few years, legislation concerning cell therapies has been drasti-

cally altered. In Europe, the regulation on advanced therapies (R ...