Microscope Image Processing

adjusted with a multiplicative correction factor, and a new compensated series is

computed. For example, if the loss of intensity with depth is modeled to follow

the exponential decay law, then the procedure for attenuation correction is as

follows [52]. A reference 3-D stack of N images is taken of a representative area

in the target specimen at a similar position, depth, and exposure as for each slice in

the sample 3-D image. The sequence number of each image (k ¼ 1,..., N) is

proportional to the total integration time. The images in the reference stack are

background corrected. An exponential decay curve, ln I

k

ðÞ¼ln I

1

ðÞlk,wherel

is the decay rate and I

1

and I

k

are the fluorescence intensities of the first and the kth

image, respectively, is fitted ...