Microscope Image Processing

by the focused image Fig. 17.4a for each of the three phases. Figure 17.4c shows

illumination for one phase after the blurring for depth has been simulated.

The square of the difference between two depth-blurred illuminations,

corresponding to I

1

 I

2

ðÞ

2

in Eq. 17.5, is shown in Fig. 17.4d. When three

such results are combined, as in Eq. 17.5, the optical section of Fig. 17.4f results.

It is also possible to construct the conventional image, shown in Fig. 17.4e, from

the three phase images by computing

I

conventional

¼ I

1

þ I

2

þ I

3

(17:8)

The earlier statement about the image containing a focused component and

a defocused one can be substantiated by comparing ...