Microscope Image Processing

signal, and high-fidelity reconstruction of this signal and any derived motion

parameters, such as velocity and acceleration, is possible only if temporal

sampling is done at a rate that complies with the theory.

Establishing this rate, however, is a chicken-and-egg problem. Before sam-

pling, one must have knowledge of the expected velocities to be estimated, which

can only be obtained by sampling at or above the proper rate in the first place.

In practice, a series of experiments at different sampling rates is often necessary

to arrive at the optimal setting. Several studies can be found in the literature

[11–13] that discuss temporal resolution for specific applications. They clearly

demonstrate how undersampling may have a significant effect on velocity ...