Microscope Image Processing

There are two methods to implement segmentation of 3-D images. The first

approach is slice by slice, in which the 2-D images in the 3-D stack are individu-

ally processed, and the 3-D regions are extracted when the processed 2-D slices

are stacked. The disadvantage of this implementation is that discontinuities of

volume between the slices may occur, and thus it is not possible to derive

accurate 3D-volumetric morphology. Object boundaries that lie parallel to the

focal plane cannot be extracted accurately.

The second approach is the volume-oriented approach, which is based on

processing the complete set of consecutive slices, in its entirety, as a