Microscope Image Processing

new estimate. Circular symmetry can be enforced on the psf by averaging the

values equidistant from the optical axis. The frequency band-limit constraint is

imposed by setting to zero all values of the Fourier transform of

^

h

kþ1

x, y, zðÞ

that lie above the cutoff frequency.

In 3-D microscopy, lateral and axial resolutions are usually different, and the

psf can be adjusted accordingly. Blind deconvolution methods take twice as long

per iteration as the ML technique because two functions (i.e., object and psf) are

being estimated. They can produce better results than using a theoretical psf,

especially if unpredicted aberrations are present. The algorithm adjusts the psf

to fit the data and can thus partially correct for spherical aberration. Blind

deconvolution ...