Microscope Image Processing

time-lapse imaging. To avoid confusion, it is better to use the abbreviations 2-D

and 3-D to refer to spatial dimensionality only and to indicate explicitly whether

the data also involves a temporal or spectral coordinate. Therefore, in this

chapter we indicate spatially 2-D and 3-D time-lapse imaging by 2-Dþt and

3-Dþt, respectively, rather than by 3-D and 4-D.

Regardless of the imaging technique being used, a careful design of the

microscope setup is imperative, because shortcomings may require additional

pre- or postprocessing of the resulting image data or may even lead to artifacts

that cannot be removed and that hamper data analysis. We include ...