Microscope Image Processing

particles, to detect particle disappearance, and to handle closely passing

particles. Experiences with an alternative but related Bayesian filtering algo-

rithm [81] applied to the tracking of cytoplasmic and nuclear HIV-1 complexes

can be found in [99].

15.7 Summary of Important Points

In this chapter we have sketched the current state of the art in time-lapse

microscopy imaging, in particular cell and particle tracking, from the perspective

of automated image processing and analysis. While it is clear that biological

investigation is relying increasingly on computerized methods, it also follows

that such methods are still very much in their infancy. Although there is

a growing consensus about the strengths and weaknesses of specific approaches,

there ...