April 2016
Beginner to intermediate
1036 pages
33h 39m
English
book
Statistics and Data Analysis for Microarrays Using R and Bioconductor, 2nd Edition
by Sorin Draghici
Content preview from Statistics and Data Analysis for Microarrays Using R and Bioconductor, 2nd Edition
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Analysis and visualization tools 523
read on the control array is 1,000 and 30,000, respectively.
3
Let us assume
that in a trea ted animal, both genes go up 1.5 times to 1,500 and 45,000,
respectively. Let us also assume that the error in reading the intensity values
is ±100. The following table shows how the individual intensities will vary.
Untreated sample Treated sample
actual r ead value r e ad
1000 900 − 1100 1500 140 0 − 1600
30000 29900 − 30100
45000 44900 − 45100
However, if we c alculate ratios between such intensity, the variance will
increase (see also Chapter 8). The lowest possible ratios will be obtained by
combining the lowest possible
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