Cell and Material Interface

124 Cell and Material Interface

shown in Figure 5.10. They also showed that stencil could be used for the deposition

of both single and multiple proteins. To achieve patterning of multiple proteins, sten-

cils are stacked up in layers and each protein deposition is followed by treatment with

antifouling agent before the rst stencil layer is removed. Incubation with antifoul-

ing agent ensures that secondary deposition of proteins in same area does not occur

unless driven by specic interaction. As seen in Figure 5.10A and B, two stencil

layers with 30 and 600 μm diameter holes were used to pattern BSA-AlexaFluor™

488 and BSA-AlexaFluor™ 555, respectiv ...