
225Polysaccharide-Based Biomaterials for Cell–Material Interface
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FIGURE 9.4 Confocal images of (a) neural network (scale bar = 300 μm) and (b) repre-
sentative neuron morphology with color band, indicating the depth of the neurite location
(scale bar = 50 μm) on chitosan–agarose hydrogels. (Reprinted with permission from Cao,
Z., Gilbert, R.J., and He, W., Simple agarose-chitosan gel composite system for enhanced
neuronal growth in three dimensions, Biomacromolecules, 10, 2954–2959. Copyright 2009
American Chemical Society.)