
139Protein Micropatterning Techniques for Tissue Engineering
during the culture. This device design allowed for dynamic control of cell interac-
tion through control of proximity of the two cell types with respect to each other.
As described in Section 5.3.2.2, a multilayered stamp can be used to sequentially
microcontact print multiple proteins for creating co-culture systems (Figure 5.21A)
[49]. Similarly, other soft lithography techniques such as microuidics-based pat-
terning and stencil-based patterning can be used to create patterned co-cultures.
When applying microuidic-based strategies, multiple isolated channels can be con-
tained in a ...