Cell and Material Interface

139Protein Micropatterning Techniques for Tissue Engineering

during the culture. This device design allowed for dynamic control of cell interac-

tion through control of proximity of the two cell types with respect to each other.

As described in Section 5.3.2.2, a multilayered stamp can be used to sequentially

microcontact print multiple proteins for creating co-culture systems (Figure 5.21A)

[49]. Similarly, other soft lithography techniques such as microuidics-based pat-

terning and stencil-based patterning can be used to create patterned co-cultures.

When applying microuidic-based strategies, multiple isolated channels can be con-

tained in a ...