April 2022
Beginner to intermediate
486 pages
10h 22m
Chinese
Content preview from 云端基因组学
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撰写可完全复现的论文
｜
449
最后，你需要用如下命令将镜像推到
Dockerhub
、
Quay.io
或
GCR
：
$ docker push <username>/<repo>:<tag>
镜像创建过程可能差别较大，具体取决于你往镜像安装什么工具。很多其他
Dockerfile
指令我们这里并未介绍，例如，你可以用
CP
命令，复制本地文件到
容器，详见上述文章链接。我们建议你在编写
Dockerfile
文件之前，先找一个
规格相似且已装到
Docker
镜像的工具，并以其
Dockerfile
为模板编写自己的
指令。你要是使用一个恰当的基础镜像，通常你自己应该不需要安装太多东西。
我们用补充材料所示
Dockerfile
创建容器镜像，接着将其发布到
Docker Hub
公有库。
为了在我们的
Terra
工作区运行工作流，我们配置它，使其在受试表的行上运行，
就像第一个工作流那样。我们用上个工作流的输出填充
sampleVcf
列，作为第二个
工作流的输入。最后，我们做好配置，将所得的两个输出文件（
BAM
和
VCF
文件）
及其索引文件写入表格的新列
synthExomeBam
和
original
-
CallsVcf
中。
该工作流就像我们第一个工作流一样也有几处效率低的地方，部分原因在于我们不
得不加清洗任务。其运行时间跟第一个工作流时间差不多，尽管在这么长时间内，
它确实做了更多工作：它处理每个受试，生成一个完整外显子组
BAM
文件，其覆
盖度和相关度量标准均参照论文预印本所报告的原法洛四联症群体的外显子组。我
们用
IGV
浏览器打开几个
BAM ...
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O’Reilly covers everything we've got, with content to help us build a world-class technology community, upgrade the capabilities and competencies of our teams, and improve overall team performance as well as their engagement.Julian F.
I wanted to learn C and C++, but it didn't click for me until I picked up an O'Reilly book. When I went on the O’Reilly platform, I was astonished to find all the books there, plus live events and sandboxes so you could play around with the technology.Addison B.
I’ve been on the O’Reilly platform for more than eight years. I use a couple of learning platforms, but I'm on O'Reilly more than anybody else. When you're there, you start learning. I'm never disappointed.Amir M.
I'm always learning. So when I got on to O'Reilly, I was like a kid in a candy store. There are playlists. There are answers. There's on-demand training. It's worth its weight in gold, in terms of what it allows me to do.